Rafael Nadal will be out for four to six weeks due to a stress fracture in his rib.
The injury occurred during the Spaniard's semi-final victory over Carlos Alcaraz at Indian Wells on Saturday.
As he lost the final against Taylor Fritz, the 21-time Grand Slam champion found breathing difficult and said he was disoriented.
While the French Open begins on May 22 in Paris, 35-year-old Nadal is scheduled to miss both of next month's clay court majors in Monte Carlo and Barcelona.
Nadal has won the Roland Garros title a total of 13 times.
He stated he "immediately went to visit my medical team to do tests after I played with discomfort in the final" when he returned to Spain, according to a post on social media.
The Spaniard added: "As it turns out, I have a stress crack in one of my ribs. This is not good news and I did not expect this."
This year, Nadal has been the best player in the world, capped by his victory at the Australian Open, when he defeated Daniil Medvedev in a thrilling final.
His loss against Fritz in California was his first of the year, and it ended a 20-match winning streak.
Nadal isn't expected to be able to practice for another four weeks, and his next competition is the Madrid Open, which starts in six weeks.
